FUNCTIONAL CHARACTERISATION OF PROTEINS

- In silico modelling of recombinant proteins for optimization of their production in plants, including post-translational modifications and prediction of subcellular localisationAnalysis of:

- Folding: resistance to proteolytic digestion, disulfide bond pattern, solubility, binding to conformation-dependent antibodies.

- Oligomerization: velocity gradient centrifugation in different biochemical conditions; dynamic light scattering (DLS); SEC-MALS.

- Glycosylation: analysis with glycan-specific antibodies and deglycosylating enzymes; mass-spectrometry analysis (glycomics)

  • Targeting and sorting: density gradient subcellular fractionation; fluorescence microscopy
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