FUNCTIONAL CHARACTERISATION OF PROTEINS

- In silico modelling of recombinant proteins for optimization of their production in plants, including post-translational modifications and prediction of subcellular localisationAnalysis of:

- Folding: resistance to proteolytic digestion, disulfide bond pattern, solubility, binding to conformation-dependent antibodies.

- Oligomerization: velocity gradient centrifugation in different biochemical conditions; dynamic light scattering (DLS); SEC-MALS.

- Glycosylation: analysis with glycan-specific ...

Enzyme/protein engineering: CELL BIOLOGY EDUCATED PROTEIN DESIGN AND TESTING

DESIGN strategies to produce high levels of recombinant proteins and peptides in plants as bioreactors according to an in silico pipeline

The known structural features of the protein of interest (POI) are used for in silico modelling of the POI, and the choices of vector, target sequence, codon-usage, expression system and purification strategy are tailored to the required POI properties (e.g. stability, purity, or alleviation of allergenic/toxic features).

TESTING the different strategies by ...

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