CNR

Biochemical characterization and modification of Carbohydrate Active Enzymes (CAZymes), especially from (hyper)thermophilic microorganisms, for their exploitation in lignocellulosic biomasses and waste products hydrolysis and valorisation Biochemical characterization of protein molecules from different natural sources, including cold-adapted proteins from polar organisms. Biochemical characterization of innovative biocatalysts involved the carbon cycle for CO2 sequestration processes and the ...

DESIGN strategies to produce high levels of recombinant proteins and peptides in plants as bioreactors according to an in silico pipeline

The known structural features of the protein of interest (POI) are used for in silico modelling of the POI, and the choices of vector, target sequence, codon-usage, expression system and purification strategy are tailored to the required POI properties (e.g. stability, purity, or alleviation of allergenic/toxic features).

TESTING the different strategies by ...

- In silico modelling of recombinant proteins for optimization of their production in plants, including post-translational modifications and prediction of subcellular localisationAnalysis of:

- Folding: resistance to proteolytic digestion, disulfide bond pattern, solubility, binding to conformation-dependent antibodies.

- Oligomerization: velocity gradient centrifugation in different biochemical conditions; dynamic light scattering (DLS); SEC-MALS.

- Glycosylation: analysis with glycan-specific ...

Identification, isolation, purification and modification of Carbohydrate Active Enzymes (CAZymes), especially from (hyper)thermophilic microorganisms, for their exploitation in lignocellulosic biomasses and waste products hydrolysis and valorization Isolation, identification and purification of protein molecules from different natural sources, including cold-adapted proteins from polar organisms. Innovative biocatalysts involved the carbon cycle for CO2 sequestration processes and the production ...

Based on the following:

-       Extraction of genomic DNAs directly from the environmental samples;

-       Preparation of metagenomic libraries;

-       Shotgun metagenomic sequencing by Next generation sequencing using Illumina technology (Metagenomics);

-       Bioinformatics analyses (meta-assembly, gene findings and annotation, metabolic reconstruction) and data delivery;

-       16S rRNA and other target genes sequencing by Next generation sequencing using Illumina technology (Metabarcoding) ...